gene pix microarray scanner Search Results


gene pix microarray scanner  (Danaher Inc)
94
Danaher Inc gene pix microarray scanner
Gene Pix Microarray Scanner, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/GenePix+Microarray+Systems/10__3126_slash_ijls__v6i1__6059-53-8-12
Average 94 stars, based on 1 article reviews
gene pix microarray scanner - by Bioz Stars, 2026-09
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imagene 7.0 software  (BioDiscovery Inc)
90
BioDiscovery Inc imagene 7.0 software
Imagene 7.0 Software, supplied by BioDiscovery Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/imagene+8+0+software/pmc03807239-38-26-29
Average 90 stars, based on 1 article reviews
imagene 7.0 software - by Bioz Stars, 2026-09
90/100 stars
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tubes  (Eppendorf AG)
99
Eppendorf AG tubes
Tubes, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/Tubes/pmc04157593-408-12-8
Average 99 stars, based on 1 article reviews
tubes - by Bioz Stars, 2026-09
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centrifuge  (Eppendorf AG)
99
Eppendorf AG centrifuge
Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/Eppendorf/pmc04157593-408-0-8
Average 99 stars, based on 1 article reviews
centrifuge - by Bioz Stars, 2026-09
99/100 stars
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anti-ubiquitin antibody  (Biomol GmbH)
90
Biomol GmbH anti-ubiquitin antibody
A. An example of a portion of one developed microarray is depicted. Different proteins and their signal on the chip are presented. B. Reproducibility of the assay. Microarrays were incubated with mitotic extracts. The poly-ubiquitination signal <t>(FK1</t> antibody, Biomol) per protein was calculated by the mean values of its spots reactivities. The reactivity level from two chips developed on different days were plotted in order to compare the variability between two biological replicates.
Anti Ubiquitin Antibody, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/anti+ubiquitin/pmc04157593-224-24-30
Average 90 stars, based on 1 article reviews
anti-ubiquitin antibody - by Bioz Stars, 2026-09
90/100 stars
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hrp conjugated goat a-mouse igg antibody  (Promega)
90
Promega hrp conjugated goat a-mouse igg antibody
A. An example of a portion of one developed microarray is depicted. Different proteins and their signal on the chip are presented. B. Reproducibility of the assay. Microarrays were incubated with mitotic extracts. The poly-ubiquitination signal <t>(FK1</t> antibody, Biomol) per protein was calculated by the mean values of its spots reactivities. The reactivity level from two chips developed on different days were plotted in order to compare the variability between two biological replicates.
Hrp Conjugated Goat A Mouse Igg Antibody, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/hrp+conjugated+secondary+antibodies/pm37708854-183-47-55
Average 90 stars, based on 1 article reviews
hrp conjugated goat a-mouse igg antibody - by Bioz Stars, 2026-09
90/100 stars
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a. coluzzii mosquitoes, ngousso strain  (BEI Resources)
90
BEI Resources a. coluzzii mosquitoes, ngousso strain
A. An example of a portion of one developed microarray is depicted. Different proteins and their signal on the chip are presented. B. Reproducibility of the assay. Microarrays were incubated with mitotic extracts. The poly-ubiquitination signal <t>(FK1</t> antibody, Biomol) per protein was calculated by the mean values of its spots reactivities. The reactivity level from two chips developed on different days were plotted in order to compare the variability between two biological replicates.
A. Coluzzii Mosquitoes, Ngousso Strain, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/a++coluzzii+mosquitoes++ngousso+strain/pm37708854-183-116-118
Average 90 stars, based on 1 article reviews
a. coluzzii mosquitoes, ngousso strain - by Bioz Stars, 2026-09
90/100 stars
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aaf 1002x miseq reagent kit v2 300 cycles  (Illumina Inc)
99
Illumina Inc aaf 1002x miseq reagent kit v2 300 cycles
A. An example of a portion of one developed microarray is depicted. Different proteins and their signal on the chip are presented. B. Reproducibility of the assay. Microarrays were incubated with mitotic extracts. The poly-ubiquitination signal <t>(FK1</t> antibody, Biomol) per protein was calculated by the mean values of its spots reactivities. The reactivity level from two chips developed on different days were plotted in order to compare the variability between two biological replicates.
Aaf 1002x Miseq Reagent Kit V2 300 Cycles, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/MiSeq+Reagent+Kit+v2/pm37708854-183-153-154
Average 99 stars, based on 1 article reviews
aaf 1002x miseq reagent kit v2 300 cycles - by Bioz Stars, 2026-09
99/100 stars
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resource source identifier antibodies goat polyclonal anti gfp rockland immunochemicals  (Rockland Immunochemicals)
96
Rockland Immunochemicals resource source identifier antibodies goat polyclonal anti gfp rockland immunochemicals
A. An example of a portion of one developed microarray is depicted. Different proteins and their signal on the chip are presented. B. Reproducibility of the assay. Microarrays were incubated with mitotic extracts. The poly-ubiquitination signal <t>(FK1</t> antibody, Biomol) per protein was calculated by the mean values of its spots reactivities. The reactivity level from two chips developed on different days were plotted in order to compare the variability between two biological replicates.
Resource Source Identifier Antibodies Goat Polyclonal Anti Gfp Rockland Immunochemicals, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/ANTI-GREEN+FLUORESCENT+PROTEIN+(GFP%2C+RE/pm37708854-183-2-9
Average 96 stars, based on 1 article reviews
resource source identifier antibodies goat polyclonal anti gfp rockland immunochemicals - by Bioz Stars, 2026-09
96/100 stars
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3724s  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc 3724s
A. An example of a portion of one developed microarray is depicted. Different proteins and their signal on the chip are presented. B. Reproducibility of the assay. Microarrays were incubated with mitotic extracts. The poly-ubiquitination signal <t>(FK1</t> antibody, Biomol) per protein was calculated by the mean values of its spots reactivities. The reactivity level from two chips developed on different days were plotted in order to compare the variability between two biological replicates.
3724s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pix+microarray+scanner/HA-Tag+Rabbit+mAb/pm37708854-183-22-18
Average 99 stars, based on 1 article reviews
3724s - by Bioz Stars, 2026-09
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Image Search Results


A. An example of a portion of one developed microarray is depicted. Different proteins and their signal on the chip are presented. B. Reproducibility of the assay. Microarrays were incubated with mitotic extracts. The poly-ubiquitination signal (FK1 antibody, Biomol) per protein was calculated by the mean values of its spots reactivities. The reactivity level from two chips developed on different days were plotted in order to compare the variability between two biological replicates.

Journal: Current protocols in protein science / editorial board, John E. Coligan ... [et al.]

Article Title: Post-Translational Modification Profiling – a high-content assay for identifying protein modifications in mammalian cellular systems

doi: 10.1002/0471140864.ps2708s77

Figure Lengend Snippet: A. An example of a portion of one developed microarray is depicted. Different proteins and their signal on the chip are presented. B. Reproducibility of the assay. Microarrays were incubated with mitotic extracts. The poly-ubiquitination signal (FK1 antibody, Biomol) per protein was calculated by the mean values of its spots reactivities. The reactivity level from two chips developed on different days were plotted in order to compare the variability between two biological replicates.

Article Snippet: TBS: 50 mM Tris-Cl 150 mM NaCl Adjust pH to 7.6 TBS-T: TBS with 0.05% Tween-20 Blocking buffer (ArrayIt) Extracts as described in the SupportProtocol Detection antibody (anti-ubiquitin antibody; FK1, BioMol) Secondary antibody (fluorescently labeled) Equipment Centrifuge (for 250 to 500 mL conical vessels) Eppendorf centrifuge for 50mL tubes Cover slips (24×60mm) Forceps GenePix 4000B microarray scanner (Molecular devices) Gene Pix Pro software (Molecular Devices) Optional: ProteinArray Prospector v.5 (available at http://www.com/site/us/home/Products-and-services/aplicationsqprotein-expressio-and-analysis/biomarker-discovery/protoarray/online-tools.html ) Protocol steps It is highly recommended that before you start with this protocol you read the user's manual of the human ProtoArray by Invitrogen/Life Technologies: Blocking the array surface list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Transfer the human ProtoArray from -20°C into 4°C for 15 minutes according to the manufacturer's instructions.

Techniques: Microarray, Incubation

Examples of expected outcomes after developing the microarrays. A. An image of a developed and scanned microarray with poly-ubiquitin antibody (FK1). Over 8000 unique human proteins on a ProtoArray human protein microarray are spotted on the surface and proteins that were modified by poly-ubiquitin were detected with a fluorescently-labeled secondary antibody. Control proteins help to orient the array grid in the data analysis step and serve as positive and negative controls for different applications. B. A portion of the microarray is enlarged (white box in A) to see the reactivity in a higher magnification. C. An example of a portion of a developed microarray with non-uniform blocking. The contrast is enhanced to show the differences in background reactivity. D. An enlarged portion of the white box in C in order to show the loss of signal reactivity in the spots that are located in the bad region.

Journal: Current protocols in protein science / editorial board, John E. Coligan ... [et al.]

Article Title: Post-Translational Modification Profiling – a high-content assay for identifying protein modifications in mammalian cellular systems

doi: 10.1002/0471140864.ps2708s77

Figure Lengend Snippet: Examples of expected outcomes after developing the microarrays. A. An image of a developed and scanned microarray with poly-ubiquitin antibody (FK1). Over 8000 unique human proteins on a ProtoArray human protein microarray are spotted on the surface and proteins that were modified by poly-ubiquitin were detected with a fluorescently-labeled secondary antibody. Control proteins help to orient the array grid in the data analysis step and serve as positive and negative controls for different applications. B. A portion of the microarray is enlarged (white box in A) to see the reactivity in a higher magnification. C. An example of a portion of a developed microarray with non-uniform blocking. The contrast is enhanced to show the differences in background reactivity. D. An enlarged portion of the white box in C in order to show the loss of signal reactivity in the spots that are located in the bad region.

Article Snippet: TBS: 50 mM Tris-Cl 150 mM NaCl Adjust pH to 7.6 TBS-T: TBS with 0.05% Tween-20 Blocking buffer (ArrayIt) Extracts as described in the SupportProtocol Detection antibody (anti-ubiquitin antibody; FK1, BioMol) Secondary antibody (fluorescently labeled) Equipment Centrifuge (for 250 to 500 mL conical vessels) Eppendorf centrifuge for 50mL tubes Cover slips (24×60mm) Forceps GenePix 4000B microarray scanner (Molecular devices) Gene Pix Pro software (Molecular Devices) Optional: ProteinArray Prospector v.5 (available at http://www.com/site/us/home/Products-and-services/aplicationsqprotein-expressio-and-analysis/biomarker-discovery/protoarray/online-tools.html ) Protocol steps It is highly recommended that before you start with this protocol you read the user's manual of the human ProtoArray by Invitrogen/Life Technologies: Blocking the array surface list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Transfer the human ProtoArray from -20°C into 4°C for 15 minutes according to the manufacturer's instructions.

Techniques: Microarray, Modification, Labeling, Blocking Assay